Does chemical cross-linking with NHS esters reflect the chemical equilibrium of protein-protein noncovalent interactions in solution?
نویسندگان
چکیده
منابع مشابه
Does chemical cross-linking with NHS esters reflect the chemical equilibrium of protein-protein noncovalent interactions in solution?
Chemical cross-linking in combination with mass spectrometry has emerged as a powerful tool to study noncovalent protein complexes. Nevertheless, there are still many questions to answer. Does the amount of detected cross-linked complex correlate with the amount of protein complex in solution? In which concentration and affinity range is specific cross-linking possible? To answer these question...
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ژورنال
عنوان ژورنال: Journal of the American Society for Mass Spectrometry
سال: 2010
ISSN: 1044-0305,1879-1123
DOI: 10.1016/j.jasms.2010.06.016